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- CaV1.2 Antibodies
Invitrogen
View all (23) CaV1.2 antibodies
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Cite CaV1.2 Monoclonal Antibody (S57)
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CaV1.2 Antibody (MA5-27717) in ICC/IF
Immunofluorescent analysis of Cav1.2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:200 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200 (1 hour at RT). Images are shown as follows: (A) Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain, (B) Anti-Cav1,2 Antibody, (C) Merged image (A) DAPI (blue) nuclear stain, (B) Phall... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
MA5-27717
Applications
Tested Dilution
Publications
Western Blot(WB)
1:1,000
View 1 publication1 publication
Immunohistochemistry (Paraffin) (IHC (P))
1:1,000
-
Immunocytochemistry(ICC/IF)
1:100
-
Immunoprecipitation(IP)
1:200
-
Antibody Microarray(AM)
Assay-dependent
-
Product Specifications
Species Reactivity
Hamster,Human,Mouse,Rabbit,Rat
Published species
Not Applicable
Host/Isotype
Mouse/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S57
Immunogen
Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
Conjugate
Unconjugated
- APC
- FITC
- PE
- PerCP
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735138
Product Specific Information
1 µg/mL of MA5-27717 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 240kDa (varies with cell background due to glycosylation).
Target Information
CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by CACNA1C binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:brain class C; CaCB receptor; CACB-receptor; CACH3; CACN4; CACNA 1D; CACNL1A2; calcium channel voltage-dependent alpha1c subunit; calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit; Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; calcium channel, voltage-dependent, alpha 1C subunit; calcium channel, voltage-dependent, L type, alpha 1C subunit; DHP receptor; DHPR, alpha-1 subunit; L-type calcium channel alpha-1 subunit; long-NT; LTCC; MBC; MELC-CC; MGC120730; Mouse brain class C; neuronal voltage-gated calcium channel alpha 1C subunit; Rat brain class C; RBC; skeletal muscle-specific calcium channel; smooth muscle calcium channel blocker; Smooth muscle calcium channel blocker receptor; unnamed protein product; voltage-dependent L-type Ca2+ channel alpha 1 subunit; Voltage-dependent L-type calcium channel subunit alpha-1C; Voltage-gated calcium channel subunit alpha Cav1.2; voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit, splice variant 10*
Gene Aliases:CACB; CACH2; CACN2; CACNA1C; CACNL1A1; CaV1.2; CCHL1A1; D930026N18Rik; I79_006562; LQT8; MBC; MELC-CC; RATIVS302; TS
Entrez Gene ID:(Rabbit) 100144322, (Human) 775, (Rabbit) 100101555, (Mouse) 12288, (Chinese hamster) 100754788, (Rat) 24239
Molecular Function: 
voltage-gated ion channel
Function(s)
immune system developmentpositive regulation of cytosolic calcium ion concentrationheart developmentregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionembryonic forelimb morphogenesiscalcium-mediated signaling using extracellular calcium sourcecamera-type eye developmentregulation of insulin secretioncalcium ion transport into cytosolcardiac conductioncalcium ion transmembrane transport via high voltage-gated calcium channelcalcium ion transmembrane transportcardiac muscle cell action potential involved in contractionmembrane depolarization during cardiac muscle cell action potentialmembrane depolarization during AV node cell action potentialcell communication by electrical coupling involved in cardiac conductionregulation of heart rate by cardiac conductionregulation of ventricular cardiac muscle cell action potentialmembrane depolarization during atrial cardiac muscle cell action potentialtransportion transportcalcium ion transportcellular calcium ion homeostasissmooth muscle contractionchemical synaptic transmissionadult walking behaviorregulation of blood pressurevisual learningcalcium ion regulated exocytosisregulation of vasoconstrictioninsulin secretiongrowth hormone secretionregulation of ion transmembrane transportglucose homeostasisregulation of organ growthtransmembrane transportsmooth muscle contraction involved in micturitioncalcium ion importmembrane depolarization during action potential
Process(es)
voltage-gated calcium channel activityprotein bindingcalmodulin bindinghigh voltage-gated calcium channel activitymetal ion bindingalpha-actinin bindingvoltage-gated calcium channel activity involved in cardiac muscle cell action potentialvoltage-gated calcium channel activity involved in AV node cell action potentialion channel activityvoltage-gated ion channel activitycalcium channel activityenzyme bindingprotein domain specific bindingion channel bindingprotein phosphatase 2A binding
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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