3D Structure of the Dihydropyridine Receptor of Skeletal Muscle (2024)

Abstract

Excitation contraction coupling, the rapid and massive Ca²⁺ release under control of an action potential that triggers muscle contraction, takes places at specialized regions of the cell called triad junctions. There, a highly ordered supramolecular complex between the dihydropyridine receptor (DHPR) and the ryanodine receptor (RyR1) mediates the quasi-instantaneous conversion from T-tubule depolarization into Ca²⁺ release from the sarcoplasmic reticulum (SR). The DHPR has several key modules required for EC coupling: the voltage sensors and II-III loop in the alpha1s subunit, and the beta subunit. To gain insight into their molecular organization, this review examines the most updated 3D structure of the DHPR as obtained by transmission electron microscopy and image reconstruction. Although structure determination of a heteromeric membrane protein such as the DHPR is challenging, novel technical advances in protein expression and 3D labeling facilitated this task. The 3D structure of the DHPR complex consists of a main body with five irregular corners around its perimeter encompassing the transmembrane alpha 1s subunit besides the intracellular beta subunit, an extended extracellular alpha 2 subunit, and a bulky intracellular II-III loop. The structural definition attained at 19 Å resolution enabled docking of the atomic coordinates of structural hom*ologs of the alpha1s and beta subunits. These structural features, together with their relative location with respect to the RyR1, are discussed in the context of the functional data.

Key Words: dihydropyridine receptor, excitation-contraction coupling, transmission electron microscopy, 3D reconstruction

The process by which an action potential propagating through the muscle plasma membrane results into Ca²⁺ release from the sarcoplasmic reticulum (SR) and subsequent muscle contraction is called excitation-contraction (EC) coupling. In skeletal muscle of vertebrates this process takes place in the triad junction, where the transverse-tubule (TT) extensions of the plasma membrane, orthogonal to the main axis of the muscle fiber, run adjacent to the terminal cisternae of the SR.¹ The close apposition of the TT and SR membranes brings together the dihydropyridine receptor (DHPR) and the ryanodine receptor (RyR1), two calcium channels residing respectively in each of these two membrane systems. Upon depolarization of the plasma membrane and TTs, the DHPRs, which sense the voltage and are conformationally coupled to RyR1s, induce the RyR1s to open. This opening results in a massive release of Ca²⁺ from the SR and the sudden raise of intracellular Ca²⁺ triggers contraction of the muscle fibers.²

DHPR controls RyR1 not only during activation (EC coupling) but also at rest, as lack of DHPR results in elevated resting cytoplasmic Ca²⁺,³ and mutations in DHPR result in the malignant hyperthermia syndrome^4-6 that has been associated with dysregulation of resting cytoplasmic Ca²⁺ levels.^7,8

Role of the dihydripyridine receptor in excitation-contraction coupling of skeletal muscle

EC coupling relies on a supramolecular complex between DHPR, which is the TT voltage sensor, and the RyR1, which is the SR Ca²⁺ release channel. The distinctive spatial relationship between these two proteins was revealed using freeze fracture – shadowing electron microscopy. On the SR terminal cisternae side of the triad junction, large proteins named “feet” have been identified as RyR1. These form ordered 2D arrays, whereby RyR1s contact each other through their corners forming two or three rows along the edge of the terminal cisternae. On the TT side, groups of four particles (tetrads), identified as four DHPRs, correlated with the disposition of individual RyR1 particles on the SR. Tetrads overlap alternate RyR1s, leaving an “orphan” RyR1 in between.⁹ The tetrads of DHPR are formed by the specific interaction between each DHPR and each of the four corners of the much larger, prism-shaped hom*otetrameric RyR1, and do not form in its absence.¹⁰ Further evidence of the tight interaction between DHPR and RyR1 is the change in intra-tetrad distance when applying an effector known to change the conformation of RyR1.¹¹

The skeletal muscle DHPR is a heteropentamer formed by the α1s or CaV1.1, α2-δ1, β1a, and γ subunits.¹² CaV1.1, an L-type voltage-activated Ca²⁺ channel, acts as the voltage sensor for EC coupling.^13,14 Since its Ca²⁺ channel activity is irrelevant in the EC coupling context,^15,16 much effort has been dedicated to understand how the DHPR transmits the voltage-sensing signal induced by membrane depolarization to the RyR1. The main candidates that have emerged are the II-III loop of a1s and the β1a subunit: their presence is essential for EC coupling^17-19 and tetrad formation.^20,21 Thus, it became important to see how these two entities are organized within the DHPR complex and how is their spatial relationship with respect to the RyR1.

Challenges in the structural determination of the DHPR

The DHPR has been visualized within the context of the muscle tissue by freeze fracture followed by rotary shadowing as described above. In this technique, the frozen tissue is fractured through the less cohesive membrane planes, exposing the protruding regions of membrane proteins. These protrusions can then be highlighted by heavy metal directional or rotary shadowing and then imaged on the transmission electron microscope (TEM).⁹ The particles however are featureless and the resolution of this technique is limited by the size of the grain of evaporated heavy metal, to around 50 Å. Additionally, it is unclear which part of the molecule is represented by the “particle” since the particle size is the same in presence and absence of the α2-δ1 subunits even though these constitute about half the molecular weight of the whole complex.²²

An approach to get higher resolution, at the expense of losing the triad context, has been to extract the DHPR complex from the membrane using detergent, and then to image the solubilized protein using TEM and single-particle 3D reconstruction. Purification and 3D structural determination of membrane proteins has many challenges when compared to soluble proteins,²³ therefore structures of membrane proteins account for less than 2% of all protein structures available in the 3D structure databases. Extraction of the protein from the membrane needs the right amount of detergent: too little detergent lowers the yield of protein, and too much detergent may denature the protein. The choice of detergent is also important; digitonin has been the most widely used for DHPR. After extraction, the DHPR is purified from the other membrane proteins using chromatography that may be further combined with size separation techniques (gel filtration, sucrose gradient). Since DHPR is a heteropentamer, the purification method also has to ensure that all subunits are present at the end of the purification process. Simultaneous heterologous expression of all DHPR subunits yielded functional DHPRs in CHO cells,²⁴ however producing sufficient quantity for protein purification could prove difficult. To overcome many of these challenges, one successful approach was to genetically engineer one of the DHPR subunits with a tag for purification, and a second tag for 3D identification. A transgenic mouse expressing this construct was then generated for protein purification.²⁵ For 3D structural determination the purified DHPR in solution is then prepared for negative staining or cryoEM, and imaged on the TEM. Negative staining uses heavy metals to provide high contrast of the dehydrated protein at the expense of resolution (up to ~20 Å resolution); cryoEM has less contrast but records images of the protein in its frozen hydrated state and has the potential for higher resolution (up to near atomic resolution). However an additional complication for membrane proteins in the case of cryoEM is the presence of detergent, which reduces image contrast. Single-particle image processing uses the different views of the protein to reconstruct its original shape. This is done by finding the exact orientation in space of each DHPR “particle”, back projecting it to obtain its 3D reconstruction using algorithms related to these used in tomography, and by averaging multiple (thousands) of such particles. Finding the spatial orientation is not trivial when the 3D structure of the protein is not known; specialized algorithms and software tackle this problem.²⁶,²⁷ Important factors favoring 3D structural determination are a large size, presence of distinctive features in the protein, and conformational rigidity. While the DHPR has distinctive features and its MW of ~450 kDa is larger than most proteins, it is slightly small for single particle image processing, and has a flexible region. In this context RyR1, five times larger than DHPR and more rigid, is a much better candidate for this technique, which has enabled its 3D reconstruction to 10 Å resolution revealing secondary structure.²⁸ For DHPR, both negative staining and cryoEM have been applied; the best resolution achieved so far is 19 Å by negative staining.²⁵

Three-dimensional structure of the dihydropyridine receptor complex

The 3D structures of skeletal DHPR that have remained consistent with the increase in resolution²⁵,²⁹,³⁰ show a main globular body with an appendage emerging from it. The structural description will be centered in our negative staining 3D reconstruction, which has the highest resolution to date (19 Å) and enabled placing of the α1s and β1a subunits for the first time.²⁵ The DHPR used to obtain these images had a heterologous β1a subunit that contained an YFP to aid in 3D sub-localization and a biotin-acceptor domain tag that was used for purification; the engineered β1a subunit was expressed in transgenic mouse as indicated above. After purification from the muscle tissue, the DHPR sample was negatively stained, imaged by TEM (Fig. 1), and its 3D reconstruction was determined by single particle analysis, using around 12,000 particles. Its dimensions are 17 x 11 x 8 nm³ for the main body and an appendage that extends 7 nm from the point of attachment to the main body (Fig. 2A-B). The main body shows a flat oblong shape with five pronounced corners around its contour, and a small protrusion on its base, opposite to the side where the appendage is attached. The engineered YFP, which is directly recognizable in the structure, together with antibody labeling in this and previous works, identifies several subunits and functional regions within the complex. CaV1.1 or α1s is the largest subunit (176 kDa) and contributes the voltage-sensing feature. It has a similar molecular organization to that found in voltage sensitive K⁺ and Na⁺ channels and it is assumed that the tertiary structure is also the same;³¹ their canonical organization consists of four repeats with six transmembrane each, S1-S6, where S1-S4 form the peripheral voltage sensors, and S5-S6 from each subunit combine in the center to form the cation channel, forming an overall square prism structure with protruding corners, and a footprint of 9 x 9 nm². For each subunit, an additional pore helix between S5-S6 and an S4-S5 linker do not cross the membrane. We placed the atomic structure of a voltage gated cation channel³² in the main body of the TEM derived structure, matching two contiguous square corners separated by 11 nm (Fig. 2C-D). We attribute the slightly wider dimensions of the TEM structure to the presence of detergent surrounding the transmembrane domain, and to the lower resolution of the TEM structure. Two voltage sensors would be totally exposed to the periphery of the structure while the other two are partially exposed to the periphery and partially interacting with the rest of the main body. A characteristic of mammalian CaV channels is that the four repeated units (I-IV) are encoded in a single polypeptide chain of almost 1,900 residues.³³ This allows for differences in sequence among the four repeats, and importantly, cytoplasmic loops connecting these repeats have distinct structural and functional roles. The I-II loop (residues 334-432) has the alpha-interacting domain (AID) region (residues 357-374) that anchors the β1a subunit.³⁴,³⁵ The II-III loop (residues 671-790), longest and partially unfolded,³⁶ is an important determinant of EC coupling;¹⁷ the sequence formed by residues 720-760 is the critical sequence for EC coupling.¹⁸,³⁷ The III-IV loop (residues 1066-1117) influences CaV1.1 current but does not appear to be involved in EC coupling interactions with the RyR1,³⁸ however its importance is underscored by the fact that mutation at residue 1086 results in malignant hyperthermia.⁵ CaV1.1 has an extended C’ domain of ~500 residues; its distal part is proteolytically cleaved in vivo and in general remains associated through non-covalent interactions.³⁹ TEM images of the DHPR incubated with antibodies against the II-III loop showed that these bound to the protuberance emerging from the main body in the center of the region attributed to the transmembrane region of CaV1.1, and on the opposite side of the appendage,²⁵ indicating that this protuberance contains the II-III loop (Fig. 2C-D). This region marks the intracellular side of CaV1.1 and hence it is plausible that it also contains at least part of the neighboring II-II and III-IV loops, and the C domain¹⁷.

α2 and δ1 subunits The extracellular α2 subunit (147 kDa) was previously assigned to the appendage emerging from the main body, using antibody labeling.²⁹,³⁰ Together with the identification of the II-III loop within the CaV1.1 subunit, this establishes the orientation of the DHPR 3D structure within the triad junction. Compared with the main body of the DHPR, the size and shape of the appendage attributed to α2 is very threshold-dependent. This is indicative of variability within this region, which is compatible with its heavy glycosylation, and may also be an indicator of flexibility.

The α2 and δ1 subunits are encoded by a single gene;⁴⁰ the product is later cleaved but remains disulfide-bonded. Thus the single-pass transmembranal δ1 subunit (24 kDa) must be at the base of the α2 subunit, anchoring it to the membrane.

β1a subunit The position of the intracellular β1a subunit (56 kDa) was established by identification of the typical barrel shape corresponding to YFP (Fig. 2F), engineered at β1a’s N terminus.²⁵ This localization is compatible with previous antibody labeling.²⁹,³⁰ The atomic structure of the core region of the β2a isoform, encompassing the Src hom*ology 3 (SH3) domain and a guanylate kinase (GK) domain,³⁵ with 84% identity with β1a, was docked into the 3D reconstruction of the DHPR complex obtained by TEM taking several factors into account. The alpha-binding pocket of the β subunit, which interacts with the AID binding region within the I-II loop of α1s, was situated adjacent to α1s in a position appropriate for this inter-subunit interaction to occur. The SH3 in the N’ region of the β subunit was placed adjacent to the identifiable YFP engineered in the N’ region. Finally, the two prominent lobes of the GK domain were fit with the two outward spikes in the 3D volume of DHPR ²⁵ (Fig. 2E-F). In addition that the choice of location and orientation satisfy all the above conditions, further confidence in this model comes from the fact that the docking is close fitting when seen from all directions (see Supplemental movie)

γ subunit The γ subunit (34 kDa), with four transmembrane domains, must be in one of the empty spaces near α 1s, as it is known to interact with this subunit.⁴¹

In the context of the quaternary interactions with the RyR1, the DHPR would be facing the RyR1 across the junctional triadic cleft. On the SR side, the RyR1’s cytoplasmic domain (i.e. the foot structure) occupies 120 Å of the cleft, practically bridging the space between the two membranes. On the TT side, the II-III loop of CaV1.1 is sandwiched between the rest of the DHPR and the RyR1, in full agreement with its vital role in EC coupling.¹⁷ The β1a subunit location, adjacent to CaV1.1 and sandwiched between the TT membrane and the RyR1, with potentially large areas of interface, is compatible with its functions of trafficking CaV1.1 to the membrane⁴² and control of EC coupling,¹⁹ possibly indirectly by determining the DHPR-RyR1 association.²¹Figure 3 summarizes how the RyR1, the DHPR and its more important EC coupling domains would be located relative to each other in the context of the triad junction.

It is expected that higher resolution structures, combined with functional studies and high electron tomography, will, in the future, provide a picture of the quaternary assembly of DHPR and RyR1 at the atomic level, and enable a more thorough understanding of the EC coupling mechanism at the molecular level. Recent advancements in membrane protein stabilization (i.e. using lipid mimics or nanodisks) and in TEM (most notably the installation of direct electron detectors) should help in the single-particle 3D structural determination of the DHPR and RyR1 at much higher resolution.⁴³

Acknowledgement

This work was supported by American Heart Association grant 14GRNT19660003.

References